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Case Study 10 Case Study 9 Case Study

SENSIBILIDAD DE LAS TÉCNICAS INMUNOANALÍTICAS  3° Parte

Factors affecting sensitivity. 2 ° Part

the foregoing in case study 8, we can observe several situations that do not fit the intended requirements and feels the need to formulate some proposals or questions:

1. In a system automated can not get an optimal reproducibility in the measurement of estradiol in doses of about 30 pg / mL
2. in manual or automated systems, there are deviations in all the analytes that comprise the quality control (QC) to multiparameter the same direction.
3. As in the previous case, but only in some analytes.
4. was an increase in the concentration of the QC at all dose levels without detecting changes in the reference values \u200b\u200b(VR) for each analyte.
5. was an increase of VR and QC at all dose levels after re-calibration of the automated system or to perform a new dose-response (DR) in a manual system.
6. In a RIA system, it is possible to obtain a good reproducibility in the trial of estradiol in doses of about 30 pg / mL when trying to work with one of the protocols proposed by the manufacturer, 1 hour incubation at 37 ° C, instead of incubating 3 hours at RT, as is indicated in another protocol.
7. When the lab processes the same set of RIA or ELISA, the same brand, and you get variations of the D50 and inaccuracies in the low QC concentration throughout the year.
8. What changes in the protocol can be made to increase the sensitivity of a inmunoanalítico? Which one can make in a technique of RIA, IRMA, EIA, or ELISA? What modifications can be performed in an automated system?
9. What variations or modifications can be performed in a RIA system for a sensitivity an order of magnitude higher to quantify T4 and fT4 in samples of canine validity Does the DR curve to interpolate human specimens?
10. Can I increase the sensitivity in manual immunometric (IRMA or ELISA) for the analytes hTSH or PSA?

In the situations described, there are several factors that can modify the parameters of the DR curves in manual and / or automated. It is very important to keep in mind that the test parameters do not change undesirable deviations or inaccuracies, when using the protocol proposed by the manufacturer, because as a result changes were seen in the results of QC samples and patients.
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