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Case Study 10 Case Study 9 Case Study

SENSIBILIDAD DE LAS TÉCNICAS INMUNOANALÍTICAS  3° Parte

Factors affecting sensitivity. 2 ° Part

the foregoing in case study 8, we can observe several situations that do not fit the intended requirements and feels the need to formulate some proposals or questions:

1. In a system automated can not get an optimal reproducibility in the measurement of estradiol in doses of about 30 pg / mL
2. in manual or automated systems, there are deviations in all the analytes that comprise the quality control (QC) to multiparameter the same direction.
3. As in the previous case, but only in some analytes.
4. was an increase in the concentration of the QC at all dose levels without detecting changes in the reference values \u200b\u200b(VR) for each analyte.
5. was an increase of VR and QC at all dose levels after re-calibration of the automated system or to perform a new dose-response (DR) in a manual system.
6. In a RIA system, it is possible to obtain a good reproducibility in the trial of estradiol in doses of about 30 pg / mL when trying to work with one of the protocols proposed by the manufacturer, 1 hour incubation at 37 ° C, instead of incubating 3 hours at RT, as is indicated in another protocol.
7. When the lab processes the same set of RIA or ELISA, the same brand, and you get variations of the D50 and inaccuracies in the low QC concentration throughout the year.
8. What changes in the protocol can be made to increase the sensitivity of a inmunoanalítico? Which one can make in a technique of RIA, IRMA, EIA, or ELISA? What modifications can be performed in an automated system?
9. What variations or modifications can be performed in a RIA system for a sensitivity an order of magnitude higher to quantify T4 and fT4 in samples of canine validity Does the DR curve to interpolate human specimens?
10. Can I increase the sensitivity in manual immunometric (IRMA or ELISA) for the analytes hTSH or PSA?

In the situations described, there are several factors that can modify the parameters of the DR curves in manual and / or automated. It is very important to keep in mind that the test parameters do not change undesirable deviations or inaccuracies, when using the protocol proposed by the manufacturer, because as a result changes were seen in the results of QC samples and patients.
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SENSITIVITY OF TECHNICAL INMUNOANALÍTICAS 3 rd party

Factors affecting sensitivity. Part 1,

The concepts that turn over in the following case studies arising from experiences in developing inmunoanalíticas techniques, observation and analysis of methodological and operational problems inherent in over 1,000 professionals and users of different techniques and analysis methodologies physical laws and physicochemical inmunoanalíticos governing systems that involve components labeled.

Citing the latest video added to the Blog, we conclude that:

In science, "THE ONLY TRUTH IS THAT THERE ARE NO SACRED sacred truths"

not claim that this is interpreted as classes in physics, physical chemistry, chemical biology, immunology, biostatistics, etc., but only through basic science can "connect the dots" inmunoanalítico system and thus able to explain and find solutions to problems that are available to the professional.

The analysis of the experience is exclusively educational purposes and for the unique understanding of Ag-Ab interaction in the methodological context in practice. Guide the search and identify the limitations of such techniques, is intended to differentiate errors or operating problems in the various manual and automated systems inmunoanalíticos. Obtaining explanations and solutions to the problems mentioned above can be extrapolated to most of the different techniques based on the same foundation.

We just do a review of all current literature about the immunodiagnostic, immunoassays and other bioanalytical techniques and observe that many authors define the sensitivity or minimum value of an analyte measured in several ways.

The reality is that sensitivity is dependent on the type of system and its corresponding measurement signal, the equilibrium or affinity constant (Ka) of it or the antibodies used, the specific activity (number and capacity generating a specific signal response per unit mass of analyte or labeled antibody conjugate), as a inmunoanalítico competitive or immunometric system and also the sensitivity is associated with the error that occurred among the replicates.

The degree of error between the duplicates and the CV obtained, including also the deviations between the different techniques, methodologies and trademarks are due to:

• Factors such as the statistical distribution it belongs to the type of response. (DO, radioactivity, fluorescence, photon events from bioluminescence, chemiluminescence and electrochemiluminescence). Efficiency measurement of the response.
• own intrinsic factors inmunoanalíticos systems.
• Physical properties such as liquid handling by the type or state of the micropipette in manual and volume size, is either a manual or automated technique.
• physical and physicochemical laws governing the system inmunoanalítico. Factors affecting the equilibrium constant (Ka) of the antibodies such as temperature, pH and ionic strength. Incubation times in which technology is developed, if the primary immunoreaction arrives or not the balance, metering system, etc.
• If a competitive system is one step, sequential competitive or non-competitive system, also known as immunometric or sandwich.
• The efficiency of separation.
• The degree of miniaturization techniques.
• The range of the calibration curve.
• molar concentration of each component within the reaction.

To begin to describe the factors affecting the sensitivity of quantitative heterogeneous inmunoanalíticos systems, we begin with the analysis of point 1.

1 - Power Antibodies. The equilibrium constant (Ka) of the average intrinsic Ka antibody or in the case of polyclonal antibodies and the concentration of immunoglobulins.

The antibody is vedette inmunoanalíticos all systems must be highly specific to the molecule to identify and quantify or obtained by immunization induced in different animal species or different techniques in the case of monoclonal antibodies.

Many authors mention that the sensitivity of inmunoanalíticos systems depend on the affinity or equilibrium constant of him or antibodies, in fact it depends on the specific activity of labeled component (quantity and capacity to generate a specific signal response per unit mass of analyte or labeled antibody conjugate), the type of system and its corresponding measurement signal, as SIC or SIM is also associated, by various factors, the error occurred between the replicates of the same sample.

antibodies (the most widely used techniques is inmunoanalíticas IgG) is a glycoprotein with a MW of approximately 158,000 Dalton, consisting of 2 double chains. Each half of the immunoglobulin comprises a heavy chain a light chain and both halves are joined by disulfide bonds. The entire molecule is divided into an area with biological activity and other immunological activity.

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Case study 7 8 Next course

METHODOLOGICAL KNOWLEDGE IS ONE OF THE PILLARS OF BIOCHEMICAL OPPORTUNITIES FOR DEVELOPING AND POSITIONING

Before developing each Factors affecting sensitivity of techniques inmunoanalíticas, we must say that several of them also affect, to varying degrees, the overall accuracy and diversion of immunoassays.

the past 50 years, inmunoanalíticas techniques contributed to the progress of clinical diagnosis in the prevention, monitoring and treatment of disease, allowed us to identify and quantify a significant number of molecules in a precise and very specific concentrations low, and allowed the replacement of cumbersome techniques, extraction, and also imprecise use of laboratory animals, such as rabbits and frogs, to obtain a biological response.

In the early 1980's, with the development and application of monoclonal antibodies se amplió mucho más el conocimiento y, en las últimas dos décadas, el avance de la automatización generó nuevos paradigmas. Cada uno de los cambios aportó problemas y desconciertos asociados, se encontraron las soluciones y se llegó, inexorablemente, al progreso.

Luego se desarrollaron las técnicas de PCR, electroforesis capilar, PCR en tiempo real, tecnologías con EIA en nano-sensores, técnicas de point of care, entre otras. Más cerca en el tiempo, y en plena etapa de desarrollo, se pueden mencionar: técnicas inmunométricas para haptenos, inmuno-array, inmuno-PCR, etcétera. Todas ellas provocarán nuevos desafíos es decir, nuevos cambios paradigms.

When it seems that everything is resolved, approach new changes, new problems, new solutions and developments, new paradigms, and the success of biochemical professional dedicated to clinical biochemistry is to adapt quickly and to anticipate those changes paradigms and begin the restructuring or reengineering of laboratories and processes.
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IMMUNOASSAY - IBYME 18 and September 19, 2010 in Buenos Aires - Argentina

Foundation Institute of Experimental Biology and Medicine - Buenos Aires - Argentina, announces a new Graduate Course Immunoassay 2010 to be held Saturday and Sunday 18 and September 19 from 9:00 to 21:00.
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Course Completion IMMUNOASSAY - IBYME 12 and June 13, 2010 in the City of Buenos Aires - Argentina

Publication portal DIAGNOSTICS NEWS - June 17, 2010
(Buenos Aires) .- The course dictated Immunoassay performed on days 12 and 13 June of the IBYME was attended by 30 Argentine and foreign professionals with a wide diversity of interests but with the same purpose, the power to interpret the methodology from the ground or essence, to understand what happens inside a reaction tube regardless of the different techniques, methodologies, types of signal responses in manual or automated, trademarks or techniques developed for research purposes.
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APPLICATION OF IMMUNOASSAY IN DENTISTRY

The best definition of applicable professional marketing professionals is biochemical:

"MARKETING IS THE PROCESS DESIGN, COMMUNICATION AND DELIVER SOLUTIONS TO PROBLEMS WORTH INDIVIDUALS AND / OR ORGANIZATIONS TRYING TO MAXIMIZE THE BENEFITS OF THESE AND THE BUSINESS, thus contributing to general welfare "

different design strategies are not limited only by the size of the laboratory, the same age, specialty or professional master's in charge, if you make such or that practice or have a particular instrument.

specialize in different disciplines in a very important but to know in depth the tools to pursue and take control of their quality is above them, as explained in case study 4.

The laboratory can be large, medium or small pero, lo más importante es la velocidad con la que se ven las oportunidades y esa es la razón por la que hacemos mención a “La Sociedad del Conocimiento”

Una bala o un proyectil no causa ningún efecto, solamente lo produce la velocidad con que sale disparado. Con esto queremos significar que la velocidad o rapidez mental para ver oportunidades está por encima del tamaño del laboratorio.

Sin comprender las leyes y los fundamentos del inmunoanálisis, es muy difícil ver oportunidades vinculadas con él, simplemente se miran, pero no se ven y también es independiente si la práctica se realiza en el propio laboratorio y se terceriza, el conocimiento to enable them to exercise the right we have to have the quality control results.

We have a link on the Web "Odontodinámica" WM edits in the article about the alleged failure, through ignorance, the availability of laboratory tests related to bone metabolism. (Edited by a renowned dentist specializing in dental implants).

Now add in the folder Articles - Opportunities Several studies that link periodontal disease with sex hormones at puberty, pregnancy and menopause.
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Abundance and Gratitude

Since I began to witness the magic that is created in the space of LeaderImpact, I felt moved to continue working on me. Although my role was basically the translator, I learned a lot about myself, about what is necessary for my growth as a leader. I also learned that the simple act of translation can be an act of leadership when it is done from love, and that can have an impact in the room and in the world.

Right at the beginning of the first withdrawal, during the opening ceremony of the circle, I was impctado three powerful questions that have accompanied me since then, around me and within me. Who am? Why am I here? Where am I?

I've been "dancing" with these questions, and although I am not particularly in the answers (I wonder if it really is about finding answers, or the next question), that I have received many Regals have opened new perspectives for me, for me and for me. This process also has fed me in my spiritual journey, following the teachings offered by The Council Guide Training in Turkey.

I have spent many years of my life thinking that it was me. Now I know not. This is the Life. I'm trying to place life and all limiting beliefs just made me play it safe, play small, play not to lose. He looked at life from a perspective of scarcity. In this way, I had to protect what we had, I thought, creating, what he dreamed o. .. Action based on fear (maybe someone would call fear-based leadership).

four years ago, I decided to give this perspective and enter into an unknown space where the author of my life. Since then I've been going through different processes and experiences that have helped me raise my level of consciousness. LeaderImpact experience has helped me go further. Measure shows that I can go further determined by the day to give my last breath. Until then, I am totally committed to go for it!

My purpose in life is to be "the rain of the sun, which drains your dreams more radical and crazy, for the love and freedom in the world." I'm here to tell you that you are unique, you have a gift to give to the world, who are naturally creative, resourceful and whole. And from that belief, dream move, as wildly as you can, around the world. You've been dreaming for Life. Nor about you!

No limits!

We are naturally creative, resourceful and complete. Just imagine for a moment, if we are alive, it is because human beings, millions of years, found their way, despite earthquakes, volcanoes (including Iceland), ice ages, major floods, devastating epidemics, etc. What we fear in 2010?

We can create our future!

On my way to my best, I have received many gifts. I am grateful to all the people and circumstances that have helped me be who I am now, and who I'm becoming. Thanks!

I have always been open to spontaneously share my ideas and dreams. He did it in a way unconscious. Now I know my gift is giving and sharing openly, without fear of what might be lost. I am a dreamer, activator and inspiring. I am a visionary. These are also my gifts, I can always find a way, because for me there is impossible. Positivity and possibility. Also my gifts.

few months ago, I took a conscious decision. Share and give, whenever you feel the urge to do so without fear. In doing so, some think I'm too generous, I trust too much in others. But when I do, is life who is being generous and trust. It is about giving and sharing without expectations, without thinking what I can get personal. The more I go in this direction, I feel fuller, and more memorable moments build my life.

want to be a "multiplier" for you, so to dream more, you become more, you think your future for the world, alineandote with your purpose in life, why life you dreamed. And I will always feel the need, from the inner impulse. I believe in abundance in the world. The universe provides everything we need, especially when we know what we need and what we ask.

"Energy is neither created nor destroyed, only transformed." It turns out that, to put my energy to serve the world to the world, with the sole intention of being who I am, I also receive abundantly. Receiving the gift of new people I know, new opportunities that knock on my door, more dreams that give light. All this, new ways for me to learn and to live my life with purpose and meaning.

With love and respect!

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As we have mentioned in case studies 2, 3 and 4 with respect to sensitivity and the paradigm shift caused by technological progress, from technical manuals competitive (RIA), passing technical manuals by immunometric (IRMA or ELISA) and reaching the full automation has been achieved for the hormone hTSH, 100 times greater sensitivity compared to those obtained by RIA techniques of decades of 70's and 80's.

As we explained in case study 4, then the factors affecting the sensitivity that we list in case study 5 and then describe in detail, the literature supports my inability to generalize, transfer, assign and / or extrapolated to all users of the same or different methodologies Analytical Sensitivity ( Sensitivity or DMD) and the Functional Sensitivity (SF) obtained by a laboratory professional, technical, development or particular instrument where, due to n number of replicates made just for the simple analysis of statistical equations, departs the reality of the clinical laboratory.

Bibliography:

• analytical validation of a set of reagents for measuring human serum thyrotropin - Mr. Pavel Melo Cala - 2003 - National Institute of Endocrinology - Cuba
• Current Goals for performance status and serum thyrotropin ( TSH) Assays - Carole A. Spencer et al. - Clinical Chemistry 42:1 140-145 (1996)
• What does Functional Sensitivity - WA Sadler et al. - Clinical Chemistry 42, No. 12, 1996

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Case study 6 Case study 5 Case study 4

inmunoanalíticas sensitivity techniques 2 ° Part.
- Factors affecting sensitivity.
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Sensitivity techniques inmunoanalíticas 1 ° parte.

- Characteristics of the sensitivity inmunoanalíticas techniques.

The word sensitivity in immunoassay adopted several definitions from the source of RIA in 1959, to date, ie more than 50 years, was the sensitivity of these techniques which led to scientists, academics, leaders opinion, professional groups, businesses, and to all who wished to express, highlight, and to designing marketing strategies of war and handling of objections about the pros and cons, advantages and disadvantages of this or methodology which, trademark, equipment, working conditions, laboratory and professional operators, finding clinical outcomes, and so on.
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Case 3

sensitivity analytical techniques
- General
The sensitivity of an analytical method is defined as capacity, for a given methodology, to discern or differentiate small variations in the concentration of an analyte.
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• Bibliography:
  1. Chapter 13 - Principles of Instrumental Analysis - 5 th Edition Skoog
  2. Article - Dr. Silvia Porro - Department of Chemistry II - UNQ

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Case Study 2 Case Study 1

It generates confusion among biochemists and doctors to embrace change of the reference values \u200b\u200b(VF) for hormones hLH and hFSH, at the end of the decade of the 80 techniques immunometric (IRMA) provide numerous advantages in sensitivity, specificity, reproducibility and increased detection range over competitive techniques (RIA). When everything seemed to be solved, there are changes, new problems and end an important solution for clinical diagnosis. The paradigm changed again.
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Paradigm established in the early '80s about the sensitivity between isotopic techniques (RIA ) and non-isotopic or colorimetric (EIA )
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We thank all of the nearly 400 colleagues, who since 1995 have supported us in our courses and seminars IBYME and other professional associations in the country, have given our different way into teaching thinking and true to our paradigms, to see the chemistry profession as a universe full of possibilities and sharing our vision for achieving the objectives.
We know that learning requires energy and appreciate the effort they have made, our greatest satisfaction was that the vast majority of participants have recognized our efforts to transmit knowledge and was also the path that has led them to be successful.
appreciate that store our Blog "TIME OF CHANGE IN THE BIOCHEMICAL" on their desktops and / or bookmarks and can also forward them the link to all contacts of colleagues biochemical and professionals involved with the area of \u200b\u200bhealth.
http://tideca-bioquimica.blogspot.com/

Sincerely

Eduardo E. Castellani & Pablo F. Hockl 
Website: http://www.tideca.net/

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The first time I read that e-mail that morning

was surprised. Left the office. Second coffee. What to think? I reviewed the last conversation with her. I opened it on my i-phone. It had worked! I smiled.

now see her again. I'm nervous. I reread ".. . The people at the center ... dialogue ... change ... future ...".

"The CEO will meet you at a time," he said ...

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not think it was impossible. Needed something magical. Those that volunteered, created magic. Challenged that impossible problem, talking for two days. That second morning, designed a way as possible.

Success!

Today, continue exploring beyond their limits.

all started with that question: How to be the best for the world?